ARG40169
anti-eIF3E antibody
anti-eIF3E antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes eIF3E |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eIF3E |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human eIF3E. (C-KLNQNSRSEAPN, NP_001559.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Viral integration site protein INT-6 homolog; EIF3-P48; EIF3S6; eIF3e; Eukaryotic translation initiation factor 3 subunit 6; eIF3-p46; INT6; Eukaryotic translation initiation factor 3 subunit E; eIF-3 p48 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P60228 Human Eukaryotic translation initiation factor 3 subunit E |
|---|---|
| Gene Symbol | EIF3E |
| Gene Full Name | eukaryotic translation initiation factor 3, subunit E |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus, PML body. [UniProt] |
| Calculated MW | 52 kDa |
Images (1) Click the Picture to Zoom In
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ARG40169 anti-eIF3E antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG40169 anti-eIF3E antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
