ARG57865

anti-eIF2 alpha phospho (Ser51) antibody

anti-eIF2 alpha phospho (Ser51) antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes eIF2 alpha phospho (Ser51)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eIF2 alpha
Antigen Species Human
Immunogen Phospho specific peptide around Ser51 of Human eIF2 alpha.
Conjugation Un-conjugated
Alternate Names eIF-2alpha; EIF-2A; Eukaryotic translation initiation factor 2 subunit 1; EIF2; EIF-2alpha; EIF2A; eIF-2-alpha; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2A; EIF-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IP1:50 - 1:100
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa + CA

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13665 Mouse EIF2S1

GeneID: 1965 Human EIF2S1

GeneID: 54318 Rat EIF2S1

Gene Symbol EIF2S1
Gene Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Background The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Function Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProt]
Cellular Localization Cytoplasmic granule. [UniProt]
Calculated MW 36 kDa
PTM Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG57865 anti-eIF2 alpha phospho (Ser51) antibody WB image

    Western blot: 25 µg of HeLa cells untreated or treated by Calyculin A (100 nM for 30 min after serum-starvation overnight). The blots were stained with ARG57865 anti-eIF2 alpha phospho (Ser51) antibody at 1:2000 dilution.

  • ARG57865 anti-eIF2 alpha phospho (Ser51) antibody IP image

    Immunoprecipitation: 200 µg extracts of HeLa cells treated by Calyculin A. The blots were immunoprecipitated and stained with ARG57865 anti-eIF2 alpha phospho (Ser51) antibody at 1:1000 dilition.