ARG57723
anti-eIF2 alpha antibody
anti-eIF2 alpha antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody recognizes eIF2 alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody detects endogenous levels of eIF2 alpha and does not cross-react with related proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | eIF2 alpha |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human eIF2 alpha protein fragments expressed in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | eIF-2alpha; EIF-2A; Eukaryotic translation initiation factor 2 subunit 1; EIF2; EIF-2alpha; EIF2A; eIF-2-alpha; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2A; EIF-2 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol. |
| Preservative | 0.03% Proclin300 |
| Stabilizer | 50% Glycerol |
| Concentration | 4 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EIF2S1 |
| Gene Full Name | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
| Background | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| Function | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity). [UniProt] |
Images (2) Click the Picture to Zoom In
-
ARG57723 anti-eIF2 alpha antibody ICC/IF image
Immunofluorescence: HeLa cells fixed by anhydrous methanol for 2 h at -20°C and stained with ARG57723 anti-eIF2 alpha antibody at 1:200 dilution.
-
ARG57723 anti-eIF2 alpha antibody WB image
Western blot: C2C12 cell lysate stained with ARG57723 anti-eIF2 alpha antibody at 1:1000 dilution.
