ARG58655

anti-eIF1AX antibody

anti-eIF1AX antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes eIF1AX
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eIF1AX
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 10-144 of Human eIF1AX (NP_001403.1).
Conjugation Un-conjugated
Alternate Names eIF-1A; EIF1A; Eukaryotic translation initiation factor 1A, X-chromosomal; Eukaryotic translation initiation factor 4C; eIF-4C; eIF-1A X isoform; EIF1AP1; EIF4C

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:200 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NCI-H460
Observed Size 16 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1964 Human EIF1AX

GeneID: 66235 Mouse EIF1AX

Swiss-port # P47813 Human Eukaryotic translation initiation factor 1A, X-chromosomal

Swiss-port # Q8BMJ3 Mouse Eukaryotic translation initiation factor 1A, X-chromosomal

Gene Symbol EIF1AX
Gene Full Name eukaryotic translation initiation factor 1A, X-linked
Background This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]
Function Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [UniProt]
Calculated MW 16 kDa

Images (2) Click the Picture to Zoom In

  • ARG58655 anti-eIF1AX antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG58655 anti-eIF1AX antibody at 1:100 dilution.

  • ARG58655 anti-eIF1AX antibody WB image

    Western blot: 25 µg of NCI-H460 cell lysate stained with ARG58655 anti-eIF1AX antibody at 1:1000 dilution.