ARG52271
anti-eEF1A2 phospho (Ser358) antibody
anti-eEF1A2 phospho (Ser358) antibody for Western blot and Mouse
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eEF1A2 phospho (Ser358) |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Rat, Chk, NHuPrm, Sheep, Xenopus laevis |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eEF1A2 |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | EF1A; Eukaryotic elongation factor 1 A-2; eEF1A-2; Elongation factor 1-alpha 2; STN; EIEE33; STNL; Statin-S1; HS1; EEF1AL; EF-1-alpha-2; MRD38 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | Specific for the ~50k eEF1A2 phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EEF1A2 |
| Gene Full Name | eukaryotic translation elongation factor 1 alpha 2 |
| Background | Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013). |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 50 kDa |
| PTM | Trimethylated at Lys-165 by EEF1AKMT3. |
Images (1) Click the Picture to Zoom In
-
ARG52271 anti-eEF1A2 phospho (Ser358) antibody WB image
Western blot: Mouse hippocampus lysate showing specific immunolabeling of the ~ 50k eEF1A2 protein phosphorylated at Ser 358 stained with ARG52271 anti-eEF1A2 phospho (Ser358) antibody.
Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).
.
