ARG66925

anti-c-Myc antibody [SQab22253]

anti-c-Myc antibody [SQab22253] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab22253] recognizes c-Myc
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22253
Isotype IgG
Target Name c-Myc
Antigen Species Human
Immunogen Synthetic peptide derived from Human c-Myc.
Conjugation Un-conjugated
Protein Full Name Myc proto-oncogene protein
Alternate Names c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Burkitt's lymphoma tissue

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4609 Human MYC

Swiss-port # P01106 Human Myc proto-oncogene protein

Gene Symbol MYC
Gene Full Name v-myc avian myelocytomatosis viral oncogene homolog
Background The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]
Cellular Localization Nucleus, nucleoplasm. Nucleus, nucleolus. [UniProt]
Calculated MW 49 kDa
PTM Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.

Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66925 anti-c-Myc antibody [SQab22253] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human Burkitt's lymphoma tissue stained with ARG66925 anti-c-Myc antibody [SQab22253]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).