ARG40626
anti-c-Jun antibody
anti-c-Jun antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes c-Jun |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | c-Jun |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human c-Jun. |
| Conjugation | Un-conjugated |
| Alternate Names | AP1; AP-1; Transcription factor AP-1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; Activator protein 1; c-Jun |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | JUN |
| Gene Full Name | jun proto-oncogene |
| Background | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; AP-1 early response transcription factor study antibody |
| Calculated MW | 36 kDa |
| PTM | Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG40626 anti-c-Jun antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40626 anti-c-Jun antibody.
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ARG40626 anti-c-Jun antibody WB image
Western blot: NIH/3T3 cell lysate stained with ARG40626 anti-c-Jun antibody.
