ARG54082
anti-beta Tubulin antibody
anti-beta Tubulin antibody for ICC/IF,Western blot and Human,Mouse,Rat,Goat,Hamster,Monkey
Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody recognizes TUBB |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Goat, Hm, Mk |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | beta Tubulin |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Tubulin beta protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | OK/SW-cl.56; CDCBM6; Tubulin beta chain; M40; TUBB5; Tubulin beta-5 chain; TUBB1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.1mg/ml BSA, 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TUBB |
| Gene Full Name | tubulin, beta class I |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Highlight | Related Antibody Duos and Panels: ARG30270 Loading Control Antibody Panel (Actin, beta Tublin, Histone H3, GAPDH) Related products: beta Tubulin antibodies; beta Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody |
| Calculated MW | 50 kDa |
| PTM | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
Images (2) Click the Picture to Zoom In
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ARG54082 anti-beta Tubulin antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54082 anti-beta Tubulin antibody at 1:500 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54082 anti-beta Tubulin antibody WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG54082 anti-beta Tubulin antibody at 1:10000 dilution.
Customer's Feedback
Excellent
Review for anti-beta Tubulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-beta Tubulin antibody
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-beta Tubulin antibody
Application：WB
Sample：A: Mouse brain, B: Rat barin, C: U87-MG
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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