ARG10206
anti-beta 2 Microglobulin antibody [D2E9]
anti-beta 2 Microglobulin antibody [D2E9] for ELISA,Flow cytometry,ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [D2E9] recognizes Human beta 2 microglobulin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | D2E9 |
| Isotype | IgG1, kappa |
| Target Name | beta 2 Microglobulin |
| Antigen Species | Human |
| Immunogen | Highly purified beta 2 microglobulin from human urine |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-2-microglobulin |
Application Instructions
| Application Note | ELISA: Clone D2E9 can be used as capturer in Sandwich ELISA for B2MG detection. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | B2M |
| Gene Full Name | beta-2-microglobulin |
| Background | Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1). It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets. B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases. Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions. |
| Function | Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30078 Beta 2 Microglobulin ELISA Antibody Duo Related products: beta 2 Microglobulin antibodies; beta 2 Microglobulin ELISA Kits; beta 2 Microglobulin Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody |
| Calculated MW | 14 kDa |
| PTM | Glycation of Ile-21 is observed in long-term hemodialysis patients. |
Images (3) Click the Picture to Zoom In
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ARG10206 anti-beta 2 Microglobulin antibody [D2E9] WB image
Western Blot: 1. B2MG antigen (100 ng) 2. Jurkat cell lysate stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) at 1µg/mL
The molecular weight of B2MG is about 13-14KD -
ARG10206 anti-beta 2 Microglobulin antibody [D2E9] Flow Cytometry image
Flow Cytometry: HeLa cells stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) followed by incubation with fluorescence labelled secondary antibody.
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ARG10206 anti-beta 2 Microglobulin antibody [D2E9] ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with anti-beta 2 Microglobulin antibody [D2E9] (ARG10206) (green)
