ARG58252

anti-alpha amylase antibody

anti-alpha amylase antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes alpha amylase
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name alpha amylase
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 242-511 of Human alpha amylase (NP_000690.1).
Conjugation Un-conjugated
Alternate Names 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY1; EC 3.2.1.1; Alpha-amylase 1; PA; AMY2; HXA; AMY2; AMY3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat intestine
Observed Size 50 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol AMY1A; AMY2A; AMY2B
Gene Full Name amylase, alpha 1A (salivary); amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic)
Background Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Cellular Localization Secreted. [UniProt]
Calculated MW 58 kDa

Images (2) Click the Picture to Zoom In

  • ARG58252 anti-alpha amylase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver injury stained with ARG58252 anti-alpha amylase antibody at 1:100 dilution.

  • ARG58252 anti-alpha amylase antibody WB image

    Western blot: 25 µg of Rat intestine lysate stained with ARG58252 anti-alpha amylase antibody at 1:1000 dilution.