ARG56134
anti-alpha Fetoprotein antibody
anti-alpha Fetoprotein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes alpha Fetoprotein |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | alpha Fetoprotein |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (14 aa) within aa. 340-390 of Human alpha Fetoprotein. |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human liver tissue lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFP |
| Gene Full Name | alpha-fetoprotein |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008] |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
Images (5) Click the Picture to Zoom In
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ARG56134 anti-alpha Fetoprotein antibody WB image
Western blot: 20 µl of HepG2 and Mouse liver lysates stained with ARG56134 anti-alpha Fetoprotein antibody at 1 µg/ml dilution.
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ARG56134 anti-alpha Fetoprotein antibody ICC/IF image
Immunofluorescence: Rat liver tissue stained with ARG56134 anti-alpha Fetoprotein antibody at 20 µg/ml dilution.
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ARG56134 anti-alpha Fetoprotein antibody IHC-P image
Immunohistochemistry: Mouse liver tissue stained with ARG56134 anti-alpha Fetoprotein antibody at 2.5 µg/ml dilution.
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ARG56134 anti-alpha Fetoprotein antibody WB image
Western blot: fetal Human liver tissue lysate stained with ARG56134 anti-alpha Fetoprotein antibody at (A) 1 µg/ml and (B) 2 µg/ml dilution.
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ARG56134 anti-alpha Fetoprotein antibody IHC-P image
Immunohistochemistry: Rat liver tissue stained with ARG56134 anti-alpha Fetoprotein antibody at 10 µg/ml dilution.
Specific References
Chromosome territory reorganization through artificial chromosome fusion is compatible with cell fate determination and mouse development
IHC-P / Mouse
