ARG22217
anti-alpha B Crystallin antibody
anti-alpha B Crystallin antibody for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat,Bovine,Chicken
Overview
| Product Description | Rabbit Polyclonal antibody recognizes alpha B Crystallin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk |
| Tested Application | ICC/IF, IHC, WB |
| Specificity | Detects ~22kDa. Does not cross-react with αA-crystallin. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | alpha B Crystallin |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide from Human alpha B crystallin |
| Conjugation | Un-conjugated |
| Alternate Names | HSPB5; Rosenthal fiber component; Renal carcinoma antigen NY-REN-27; MFM2; Alpha; CTRCT16; B; HspB5; Heat shock protein beta-5; HEL-S-101; CTPP2; CMD1II; CRYA2; Alpha-crystallin B chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Rabbit antiserum. |
| Buffer | Rabbit Antiserum |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CRYAB |
| Gene Full Name | crystallin, alpha B |
| Background | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus |
| Calculated MW | 20 kDa |
Images (4) Click the Picture to Zoom In
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ARG22217 anti-alpha B Crystallin antibody WB image
Western blot: 20 µg of U2OS and HeLa cell lysates stained with ARG22217 anti-alpha B Crystallin antibody at 1:1000 dilution.
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ARG22217 anti-alpha B Crystallin antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22217 anti-alpha B Crystallin antibody (red) at 1:120 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22217 anti-alpha B Crystallin antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22217 anti-alpha B Crystallin antibody (green) at 1:120 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22217 anti-alpha B Crystallin antibody WB image
Western blot: 15 µg of Rat Brain cell lysates stained with ARG22217 anti-alpha B Crystallin antibody at 1:1000 for 60 min at RT. Block: 5% Skim Milk in 1X TBST. Secondary Antibody: Goat anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT.
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Review for anti-alpha B Crystallin antibody
Application：WB
Sample：Rat heart
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
