ARG22216
anti-alpha B Crystallin antibody [1A7.D5]
anti-alpha B Crystallin antibody [1A7.D5] for ELISA,ICC/IF,Western blot and Human,Rat,Bovine,Pig
Overview
| Product Description | Mouse Monoclonal antibody [1A7.D5] recognizes alpha B Crystallin |
|---|---|
| Tested Reactivity | Hu, Rat, Bov, Pig |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | Detects ~20kDa (Predicted mol. weight is ~21kDa). Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A7.D5 |
| Isotype | IgG1 |
| Target Name | alpha B Crystallin |
| Antigen Species | Human |
| Immunogen | Native Alpha B Crystallin |
| Conjugation | Un-conjugated |
| Alternate Names | HSPB5; Rosenthal fiber component; Renal carcinoma antigen NY-REN-27; MFM2; Alpha; CTRCT16; B; HspB5; Heat shock protein beta-5; HEL-S-101; CTPP2; CMD1II; CRYA2; Alpha-crystallin B chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CRYAB |
| Gene Full Name | crystallin, alpha B |
| Background | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus |
| Highlight | Related products: anti-alpha B Crystallin antibody [1A7.D5] |
| Calculated MW | 20 kDa |
Images (2) Click the Picture to Zoom In
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ARG22216 anti-alpha B Crystallin antibody [1A7.D5] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary antibody: ARG22216 anti-alpha B Crystallin antibody [1A7.D5] at 1:100 for 60 min at RT. Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Primary antibody. (D) Composite.
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ARG22216 anti-alpha B Crystallin antibody [1A7.D5] WB image
Western blot: 15 µg of Bovine cell lysates stained with ARG22216 anti-alpha B Crystallin antibody [1A7.D5] at 1:50 dilution (2 hours at RT).
