ARG45408
anti-alpha Adaptin antibody
anti-alpha Adaptin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes alpha Adaptin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | alpha Adaptin |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human alpha Adaptin. |
| Conjugation | Un-conjugated |
| Alternate Names | AP2A2; Adaptor Related Protein Complex 2 Subunit Alpha 2; HYPJ; HIP9; KIAA0899; CLAPA2; ADTAB; Clathrin-Associated/Assembly/Adaptor Protein, Large, Alpha 2; Clathrin Assembly Protein Complex 2 Alpha-C Large Chain; Plasma Membrane Adaptor HA2/AP2 Adaptin Alpha C Subunit; Alpha-Adaptin C; Huntingtin Interacting Protein J; Adaptor Related Protein Complex 2 Alpha 2 Subunit; 100 KDa Coated Vesicle Protein C; Huntingtin-Interacting Protein 9; AP-2 Complex Subunit Alpha-2; Huntingtin Yeast Partner J; Adaptin, Alpha B; Alpha2-Adaptin; DKFZP564D1864; HIP-9; Adapter-Related Protein Complex 2 Subunit Alpha-2; Adaptor-Related Protein Complex 2 Subunit Alpha-2; Adaptor Protein Complex AP-2 Subunit Alpha-2; Huntingtin-Interacting Protein J; Alpha-Adaptin C |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 104 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AP2A2 |
| Gene Full Name | Adaptor Related Protein Complex 2 Subunit Alpha 2 |
| Background | The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016] |
| Function | The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. [UniProt] |
| Cellular Localization | Cell membrane; Membrane. [UniProt] |
| Calculated MW | 104 kDa |
Images (6) Click the Picture to Zoom In
-
ARG45408 anti-alpha Adaptin antibody IHC-P image
Immunohistochemistry: Human lymphoma stained with ARG45408 anti-alpha Adaptin antibody at 2 μg/ml dilution.
-
ARG45408 anti-alpha Adaptin antibody ICC/IF image
Immunofluorescence: PC-3 stained with ARG45408 anti-alpha Adaptin antibody at 5 μg/ml dilution.
-
ARG45408 anti-alpha Adaptin antibody WB image
Western blot: HepG2 and HEK293 stained with ARG45408 anti-alpha Adaptin antibody at 0.25 μg/ml dilution.
-
ARG45408 anti-alpha Adaptin antibody FACS image
Flow Cytometry: 293T stained with ARG45408 anti-alpha Adaptin antibody at 1 µg/10^6 cells dilution.
-
ARG45408 anti-alpha Adaptin antibody WB image
Western blot: Rat brain stained with ARG45408 anti-alpha Adaptin antibody at 0.25 μg/ml dilution.
-
ARG45408 anti-alpha Adaptin antibody WB image
Western blot: Mouse brain stained with ARG45408 anti-alpha Adaptin antibody at 0.25 μg/ml dilution.
