ARG57903
anti-alpha Actinin 3 antibody
anti-alpha Actinin 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes alpha Actinin 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | alpha Actinin 3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 580-630 of Human alpha Actinin-3 (NP_001095.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; Alpha-actinin-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | LO2 and SP2/0 | ||||||
| Observed Size | ~ 103 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACTN3 |
| Gene Full Name | actinin, alpha 3 (gene/pseudogene) |
| Background | This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014] |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. [UniProt] |
| Calculated MW | 103 kDa |
Images (2) Click the Picture to Zoom In
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ARG57903 anti-alpha Actinin 3 antibody WB image
Western blot: 25 µg of LO2 cell lysaste stained with ARG57903 anti-alpha Actinin 3 antibody at 1:3000 dilution.
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ARG57903 anti-alpha Actinin 3 antibody WB image
Western blot: 25 µg of Sp2/0 cell lysaste stained with ARG57903 anti-alpha Actinin 3 antibody at 1:3000 dilution.
