ARG58210

anti-alpha 1 microglobulin antibody

anti-alpha 1 microglobulin antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes alpha 1 microglobulin
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name alpha 1 microglobulin
Antigen Species Human
Immunogen E. coli-derived Human Alpha 1 microglobulin recombinant protein (Position: A206-N352).
Conjugation Un-conjugated
Alternate Names Bikunin; HCP; ITIL; ITILC; ITI; ITI-LC; UTI; Protein HC; Complex-forming glycoprotein heterogeneous in charge; EDC1; HI30; HI-30; Alpha-1 microglycoprotein; A1M; IATIL; Uronic-acid-rich protein; Protein AMBP

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11699 Mouse AMBP

GeneID: 25377 Rat AMBP

Swiss-port # Q07456 Mouse Protein AMBP

Swiss-port # Q64240 Rat Protein AMBP

Gene Symbol AMBP
Gene Full Name alpha-1-microglobulin/bikunin precursor
Background This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]
Function Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Trypstatin is a trypsin inhibitor. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 39 kDa
PTM The precursor is proteolytically processed into separately functioning proteins.

3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.

Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.

N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG58210 anti-alpha 1 microglobulin antibody WB image

    Western blot: 50 µg of Rat liver, Mouse liver and NIH/3T3 whole cell lysates stained with ARG58210 anti-alpha 1 microglobulin antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.