ARG45432
anti-ZNRF3 antibody
anti-ZNRF3 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ZNRF3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZNRF3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human ZNRF3. |
| Conjugation | Un-conjugated |
| Alternate Names | ZNRF3; Zinc And Ring Finger 3; RNF203; BK747E2.3; KIAA1133; RING-Type E3 Ubiquitin Transferase ZNRF3; E3 Ubiquitin-Protein Ligase ZNRF3; Zinc/RING Finger Protein 3; RING Finger Protein 203; FLJ22057; Novel C3HC4 Type Zinc Finger (Ring Finger); CTA-292E10.6; EC 2.3.2.27 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 130 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ZNRF3 |
| Gene Full Name | Zinc And Ring Finger 3 |
| Background | Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in negative regulation of Wnt signaling pathway; protein ubiquitination; and ubiquitin-dependent protein catabolic process. Located in plasma membrane. [provided by Alliance of Genome Resources, Nov 2024] |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby restricting the size of the intestinal stem cell zone. [UniProt] |
| Cellular Localization | Cell membrane. [UniProt] |
| Calculated MW | 101 kDa |
Images (3) Click the Picture to Zoom In
-
ARG45432 anti-ZNRF3 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45432 anti-ZNRF3 antibody at 5 μg/ml dilution.
-
ARG45432 anti-ZNRF3 antibody WB image
Western blot: CACO-2 stained with ARG45432 anti-ZNRF3 antibody at 0.5 μg/ml dilution.
-
ARG45432 anti-ZNRF3 antibody FACS image
Flow Cytometry: PC-3 stained with ARG45432 anti-ZNRF3 antibody at 1 µg/10^6 cells dilution.
