Overview

Product Description	Rabbit Polyclonal antibody recognizes ZEB1
Tested Reactivity	Hu
Tested Application	FACS, ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ZEB1
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human ZEB1.
Conjugation	Un-conjugated
Alternate Names	Transcription factor 8; DELTAEF1; PPCD3; TCF-8; AREB6; BZP; TCF8; NIL-2-A zinc finger protein; FECD6; NIL2A; Zinc finger E-box-binding homeobox 1; Negative regulator of IL2; ZFHX1A; ZFHEP

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HEK293

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 6935 Human ZEB1 Swiss-port # P37275 Human Zinc finger E-box-binding homeobox 1
Gene Symbol	ZEB1
Gene Full Name	zinc finger E-box binding homeobox 1
Background	This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
Function	Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. [UniProt]
Calculated MW	124 kDa

Images (1) Click the Picture to Zoom In