ARG55025
anti-ZBTB7B antibody
anti-ZBTB7B antibody for ICC/IF,Western blot and Human
Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ZBTB7B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZBTB7B |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 440-469 (C-terminus) of Human ZBTB7B. |
| Conjugation | Un-conjugated |
| Alternate Names | T-helper-inducing POZ/Krueppel-like factor; CKROX; Zinc finger and BTB domain-containing protein 7B; ZBTB15; ZNF857B; ZFP-67; Zinc finger protein Th-POK; Zinc finger protein 857B; THPOK; c-KROX; Zinc finger and BTB domain-containing protein 15; ZFP67; hcKrox; hcKROX; Zinc finger protein 67 homolog; Zfp-67; Krueppel-related zinc finger protein cKrox |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | 293 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15156 Human Zinc finger and BTB domain-containing protein 7B |
|---|---|
| Gene Symbol | ZBTB7B |
| Gene Full Name | zinc finger and BTB domain containing 7B |
| Background | This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes (By similarity). [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Gene Regulation antibody; Immune System antibody |
| Calculated MW | 58 kDa |
Images (2) Click the Picture to Zoom In
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ARG55025 anti-ZBTB7B antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55025 anti-ZBTB7B antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
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ARG55025 anti-ZBTB7B antibody WB image
Western blot: 35 µg of 293 cell lysate stained with ARG55025 anti-ZBTB7B antibody.
