ARG63855

anti-ZASP / CYPHER / LDB3 antibody

anti-ZASP / CYPHER / LDB3 antibody for Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ZASP / CYPHER / LDB3
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ZASP / CYPHER / LDB3
Antigen Species Human
Immunogen C-KSQNKPEDEADE
Conjugation Un-conjugated
Alternate Names Protein cypher; Z-band alternatively spliced PDZ-motif protein; PDLIM6; CYPHER; CMPD3; CMH24; ZASP; LDB3Z4; ORACLE; LVNC3; LDB3Z1; MFM4; LIM domain-binding protein 3; CMD1C

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11155 Human LDB3

Swiss-port # O75112 Human LIM domain-binding protein 3

Background This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]
Research Area Signaling Transduction antibody
Calculated MW 77 kDa

Images (1) Click the Picture to Zoom In

  • ARG63855 anti-ZASP / CYPHER / LDB3 antibody WB image

    Western blot: Human Skeletal Muscle lysate (35 µg protein in RIPA buffer) stained with ARG63855 anti-ZASP / CYPHER / LDB3 antibody at 0.1 µg/ml dilution.