Overview

Product Description	Mouse Monoclonal antibody recognizes YY1
Tested Reactivity	Hu
Tested Application	WB
Host	Mouse
Clonality	Monoclonal
Clone	1183CT6.5.23.6
Isotype	IgG2a
Target Name	YY1
Immunogen	Purified His-tagged YY1 protein.
Conjugation	Un-conjugated
Alternate Names	YY-1; UCRBP; INO80S; NF-E1; Delta transcription factor; Yin and yang 1; DELTA; YIN-YANG-1; Transcriptional repressor protein YY1; INO80 complex subunit S

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Daudi

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS and 0.09% (W/V) Sodium azide
Preservative	0.09% (W/V) Sodium azide
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 7528 Human YY1 Swiss-port # P25490 Human Transcriptional repressor protein YY1
Gene Symbol	YY1
Gene Full Name	YY1 transcription factor
Background	YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]
Function	Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements. [UniProt]
Cellular Localization	Nucleus matrix Note=Associated with the nuclear matrix
Research Area	Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
Calculated MW	45 kDa
PTM	Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites. Ubiquitinated.

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