ARG55021
anti-YBX1 antibody
anti-YBX1 antibody for ICC/IF,Western blot and Human
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes YBX1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Bov, Rb |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | YBX1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 149-178 (Center) of Human YBX1. |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclease-sensitive element-binding protein 1; Y-box transcription factor; DBPB; YB1; CSDA2; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; MDR-NF1; BP-8; Enhancer factor I subunit A; EFI-A; YB-1; CSDB; NSEP1; Y-box-binding protein 1; NSEP-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | 293T |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P67809 Human Nuclease-sensitive element-binding protein 1 |
|---|---|
| Gene Symbol | YBX1 |
| Gene Full Name | Y box binding protein 1 |
| Function | Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus, Secreted |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 36 kDa |
| PTM | Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability. In the absence of phosphorylation the protein is retained in the cytoplasm. Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG55021 anti-YBX1 antibody ICC/IF image
Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG55021 anti-YBX1 antibody (green) at 1:25, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 hour at 37°C).
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ARG55021 anti-YBX1 antibody WB image
Western blot: 35 µg of 293T cell lysate stained with ARG55021 anti-YBX1 antibody.
