ARG57763
anti-XPA antibody [71H3]
anti-XPA antibody [71H3] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [71H3] recognizes XPA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 71H3 |
| Isotype | IgG1, kappa |
| Target Name | XPA |
| Antigen Species | Human |
| Immunogen | Recombinant protein around aa. 1-273 of Human XPA. |
| Conjugation | Un-conjugated |
| Alternate Names | XP1; XPAC; Xeroderma pigmentosum group A-complementing protein; DNA repair protein complementing XP-A cells |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P23025 Human DNA repair protein complementing XP-A cells |
|---|---|
| Gene Symbol | XPA |
| Gene Full Name | xeroderma pigmentosum, complementation group A |
| Background | This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009] |
| Function | Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. [UniProt] |
| Calculated MW | 31 kDa |
| PTM | ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. Ubiquitinated by HERC2 leading to degradation by the proteasome. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG57763 anti-XPA antibody [71H3] WB image
Western blot: 40 µg of SW480, K562, MCF7, LNCaP and Raji cell lysates stained with ARG57763 anti-XPA antibody [71H3] at 1:1000 dilution.
