ARG62661
anti-XPA antibody [12F5]
anti-XPA antibody [12F5] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [12F5] recognizes XPA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12F5 |
| Isotype | IgG2a, kappa |
| Target Name | XPA |
| Antigen Species | Human |
| Immunogen | Recombinant human XPA protein. |
| Conjugation | Un-conjugated |
| Alternate Names | XP1; XPAC; Xeroderma pigmentosum group A-complementing protein; DNA repair protein complementing XP-A cells |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Ls174T or MCF-7 cells or Human tonsil. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P23025 Human DNA repair protein complementing XP-A cells |
|---|---|
| Gene Symbol | XPA |
| Gene Full Name | xeroderma pigmentosum, complementation group A |
| Background | This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009] |
| Function | Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 31 kDa |
| PTM | ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. Ubiquitinated by HERC2 leading to degradation by the proteasome. |
Clone References
Nucleotide excision repair is not induced in human embryonic lung fibroblasts treated with environmental pollutants.
WB /
Nucleotide excision repair is associated with the replisome and its efficiency depends on a direct interaction between XPA and PCNA.
ICC/IF / Human
Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair.
WB, ICC/IF / Human
Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA.
WB / Human
Recognition of cisplatin-DNA interstrand cross-links by replication protein A.
WB / Bovine
DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.
IP / Human
Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma.
IHC / Human
