ARG52472
anti-XIRP1 phospho (Ser295) antibody
anti-XIRP1 phospho (Ser295) antibody for Western blot and Mouse
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes XIRP1 phospho (Ser295) |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Rat, Dog, NHuPrm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | XIRP1 |
| Antigen Species | Mouse |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Cardiomyopathy-associated protein 1; CMYA1; Xin; Xin actin-binding repeat-containing protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~250k and ~150k splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling of the XIRP1 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | XIRP1 |
| Gene Full Name | xin actin-binding repeat containing 1 |
| Background | XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXinα (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser295 is thought to play a key role in XIRP signaling. |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 199 kDa |
Images (1) Click the Picture to Zoom In
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ARG52472 anti-XIRP1 phospho (Ser295) antibody WB image
Western blot: Mouse heart lysate showing specific immunolabeling of the ~250k and ~150k splice variants of the XIRP1 protein phosphorylated at Ser 295 stained with ARG52472 anti-XIRP1 phospho (Ser295) antibody.
Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).
