ARG43803
anti-XCR1 / CCXCR1 antibody
anti-XCR1 / CCXCR1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes XCR1 / CCXCR1 |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | XCR1 / CCXCR1 |
Antigen Species | Human |
Immunogen | Recombinant protein corresponding to a.a. F246-N274 of Human XCR1 / CCXCR1. |
Conjugation | Un-conjugated |
Protein Full Name | Chemokine XC receptor 1 |
Alternate Names | XCR1; X-C Motif Chemokine Receptor 1; CCXCR1; GPR5; Chemokine (C Motif) XC Receptor 1; Chemokine (C Motif) Receptor 1; Chemokine XC Receptor 1; XC Chemokine Receptor 1; Lymphotactin Receptor; G Protein-Coupled Receptor 5; G-Protein Coupled Receptor 5 |
Application Instructions
Application Suggestion |
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Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Positive Control | RT4; U-87MG; HEL; U2OS; Human spleen; Rat spleen tissue; Mouse spleen; Mouse thymus tissue; Rat thymus. | ||||||
Observed Size | ~42 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
Stabilizer | 4% Trehalose |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | XCR1 |
Gene Full Name | X-C Motif Chemokine Receptor 1 |
Background | The protein encoded by this gene is a chemokine receptor belonging to the G protein-coupled receptor superfamily. The family members are characterized by the presence of 7 transmembrane domains and numerous conserved amino acids. This receptor is most closely related to RBS11 and the MIP1-alpha/RANTES receptor. It transduces a signal by increasing the intracellular calcium ions level. The viral macrophage inflammatory protein-II is an antagonist of this receptor and blocks signaling. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2020] |
Function | Receptor for chemokines SCYC1 and SCYC2. Subsequently transduces a signal by increasing the intracellular calcium ions level. Receptor for XCL1/Lymphotactin. [UniProt] |
Cellular Localization | Cell membrane; Membrane. [UniProt] |
Calculated MW | 38.5 kDa |
PTM | Disulfide bond. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG43803 anti-XCR1 / CCXCR1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43803 anti-XCR1 / CCXCR1 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43803 anti-XCR1 / CCXCR1 antibody WB image
Western blot: 30 µg of samples under reducing conditions. RT4 cells, U-87MG cells, HEL cells, U2OS cells, Rat spleen tissue, Mouse spleen tissue lysates stained with ARG43803 anti-XCR1 / CCXCR1 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG43803 anti-XCR1 / CCXCR1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded MMouse thymus tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43803 anti-XCR1 / CCXCR1 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43803 anti-XCR1 / CCXCR1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat thymus tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43803 anti-XCR1 / CCXCR1 antibody at 2 µg/ml dilution, overnight at 4°C.