ARG40182
anti-WTAP antibody
anti-WTAP antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes WTAP |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WTAP |
| Antigen Species | Human |
| Immunogen | Full length fusion protein of Human WTAP. |
| Conjugation | Un-conjugated |
| Alternate Names | Mum2; WT1-associated protein; Wilms tumor 1-associating protein; Female-lethal; 2; hFL; Pre-mRNA-splicing regulator WTAP |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Human thyroid cancer |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WTAP |
| Gene Full Name | Wilms tumor 1 associated protein |
| Background | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012] |
| Function | Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. [UniProt] |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus speckle. [UniProt] |
| Highlight | Related news: m6A reader YTHDF2 in mRNA decay and aggresome formation; |
| Calculated MW | 44 kDa |
Images (1) Click the Picture to Zoom In
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ARG40182 anti-WTAP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer stained with ARG40182 anti-WTAP antibody (left) at 1:35 dilution, or the same antibody pre-incubated with antigen (right).
