ARG66816
anti-WT1 / Wilms tumor 1 antibody [SQab20214]
anti-WT1 / Wilms tumor 1 antibody [SQab20214] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20214] recognizes WT1 / Wilms tumor 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20214 |
| Isotype | IgG |
| Target Name | WT1 / Wilms tumor 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 1-100 of Human WT1 / Wilms tumor 1. |
| Conjugation | Un-conjugated |
| Alternate Names | WIT-2; EWS-WT1; GUD; WAGR; AWT1; Wilms tumor protein; NPHS4; WT33 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WT1 |
| Gene Full Name | Wilms tumor 1 |
| Background | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015] |
| Function | Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921). [UniProt] |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190). Shuttles between nucleus and cytoplasm. Isoform 1: Nucleus speckle. Isoform 4: Nucleus, nucleoplasm. [UniProt] |
| Calculated MW | 49 kDa |
Images (1) Click the Picture to Zoom In
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ARG66816 anti-WT1 / Wilms tumor 1 antibody [SQab20214] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66816 anti-WT1 / Wilms tumor 1 antibody [SQab20214].
