ARG64032
anti-WT1 / Wilms tumor 1 antibody
anti-WT1 / Wilms tumor 1 antibody for Western blot and Human
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes WT1 / Wilms tumor 1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WT1 / Wilms tumor 1 |
| Antigen Species | Human |
| Immunogen | QDPASTCVPEPASQH |
| Conjugation | Un-conjugated |
| Alternate Names | WIT-2; EWS-WT1; GUD; WAGR; AWT1; Wilms tumor protein; NPHS4; WT33 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Oct 2010] |
| Research Area | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 49 kDa |
Images (1) Click the Picture to Zoom In
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ARG64032 anti-WT1 / Wilms tumor 1 antibody WB image
Western blot: 35 µg of Human spleen (A), kidney (B) and testis (C) lysates (in RIPA buffer) stained with ARG64032 anti-WT1 / Wilms tumor 1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
