ARG44566
anti-WHSC2 / NELF-A antibody
anti-WHSC2 / NELF-A antibody for Flow cytometry,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes WHSC2 / NELF-A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WHSC2 / NELF-A |
| Antigen Species | Human |
| Immunogen | Human WHSC2 / NELF-A recombinant protein (aa. sequence: I38-L482). |
| Conjugation | Un-conjugated |
| Alternate Names | NELFA; Negative Elongation Factor Complex Member A; NELF-A; WHSC2; Wolf-Hirschhorn Syndrome Candidate 2 Protein; Negative Elongation Factor A; Wolf-Hirschhorn Syndrome Candidate 2; P/OKcl.15 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Preservative | 4% Trehalose |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NELFA |
| Gene Full Name | Negative Elongation Factor Complex Member A |
| Background | This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008] |
| Function | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. [Uniprot] |
| Cellular Localization | Nucleus. [Uniprot] |
| Calculated MW | 57 kDa |
| PTM | Phosphoprotein. [Uniprot] |
Images (2) Click the Picture to Zoom In
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ARG44566 anti-WHSC2 / NELF-A antibody WB image
Western blot: HeLa, Jurkat and K562 stained with ARG44566 anti-WHSC2 / NELF-A antibody at 0.5 μg/mL dilution.
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ARG44566 anti-WHSC2 / NELF-A antibody FACS image
Flow Cytometry: JK stained with ARG44566 anti-WHSC2 / NELF-A antibody at 1 μg/10^6 cells dilution.
