ARG54144
anti-WDR77 antibody
anti-WDR77 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes MEP50/WDR77 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | WDR77 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human WDR77 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Nbla10071; p44/Mep50; WD repeat-containing protein 77; MEP50; HKMT1069; Methylosome protein 50; p44; MEP-50; Androgen receptor cofactor p44 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WDR77 |
| Gene Full Name | WD repeat domain 77 |
| Background | Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. |
| Function | Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. |
| Research Area | Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 37 kDa |
Images (2) Click the Picture to Zoom In
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ARG54144 anti-WDR77 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed in 1% Paraformaldehyde, permeabilized in 0.1% Triton X-100 and stained with ARG54144 anti-WDR77 antibody at 1:100 dilution.
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ARG54144 anti-WDR77 antibody WB image
Western blot: K562 cell lysate stained with ARG54144 anti-WDR77 antibody at 1:1000 dilution.
