ARG59507
anti-WASL antibody
anti-WASL antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes WASL |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WASL |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-250 of Human WASL (NP_003932.3). |
| Conjugation | Un-conjugated |
| Alternate Names | WASPB; N-WASP; NWASP; Neural Wiskott-Aldrich syndrome protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Rat brain, Mouse brain and HeLa | ||||||||
| Observed Size | 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00401 Human Neural Wiskott-Aldrich syndrome protein Swiss-port # Q91YD9 Mouse Neural Wiskott-Aldrich syndrome protein |
|---|---|
| Gene Symbol | WASL |
| Gene Full Name | Wiskott-Aldrich syndrome-like |
| Background | This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013] |
| Function | Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Note=Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59507 anti-WASL antibody WB image
Western blot: 25 µg of Rat brain, Mouse brain and HeLa cell lysates stained with ARG59507 anti-WASL antibody at 1:3000 dilution.
