ARG45086
anti-WASHC1 antibody
anti-WASHC1 antibody for Western blot,ICC/IF and Chicken,Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes WASHC1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Chk |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WASHC1 |
| Antigen Species | Human |
| Immunogen | WASH synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human WASH. This sequence is highly conserved in rat, mouse, and chicken WASH, and has low homology to other WASP family members. |
| Conjugation | Un-conjugated |
| Alternate Names | WASHC1; WASH Complex Subunit 1; WAS Protein Family Homolog 1; FAM39E; WASH1; Family With Sequence Similarity 39, Member E; CXYorf1-Like Protein On Chromosome 9; FLJ00038; Actin Nucleation Promoting Factor; FLJ00075 Protein; Protein FAM39E; WASH |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antigen Affinity Purified. |
| Buffer | PBS, 0.05% NaN3, 50% Glycerol and 0.1 % BSA. |
| Stabilizer | 50% Glycerol and 0.1 % BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WASHC1 |
| Gene Full Name | WASH Complex Subunit 1 |
| Background | Enables alpha-tubulin binding activity and ubiquitin protein ligase binding activity. Involved in several processes, including Arp2/3 complex-mediated actin nucleation; endosomal transport; and positive regulation of pseudopodium assembly. Located in early endosome. Part of WASH complex. Colocalizes with exocyst. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity. [Uniprot] |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane. [Uniprot] |
| Calculated MW | 50 kDa |
| PTM | Isopeptide bond, Ubl conjugation. [Uniprot] |
