ARG56949
anti-Visfatin antibody [4D5]
anti-Visfatin antibody [4D5] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4D5] recognizes Visfatin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D5 |
| Isotype | IgG1, kappa |
| Target Name | Visfatin |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-491 of Human Visfatin. |
| Conjugation | Un-conjugated |
| Alternate Names | Visfatin; VF; Pre-B-cell colony-enhancing factor 1; PBEF1; NAmPRTase; Nampt; EC 2.4.2.12; VISFATIN; Nicotinamide phosphoribosyltransferase; 1110035O14Rik; PBEF; Pre-B cell-enhancing factor |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P43490 Human Nicotinamide phosphoribosyltransferase |
|---|---|
| Gene Symbol | NAMPT |
| Gene Full Name | nicotinamide phosphoribosyltransferase |
| Background | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011] |
| Function | Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity). [UniProt] |
| Calculated MW | 56 kDa |
Images (3) Click the Picture to Zoom In
-
ARG56949 anti-Visfatin antibody [4D5] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56949 anti-Visfatin antibody [4D5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
-
ARG56949 anti-Visfatin antibody [4D5] WB image
Western blot: 30 ug of 293T cell lysate stained with ARG56949 anti-Visfatin antibody [4D5] at 1:1000.
-
ARG56949 anti-Visfatin antibody [4D5] WB image
Western blot: 40 µg of SW480 cell lysate stained with ARG56949 anti-Visfatin antibody [4D5] at 1:1000.
