ARG45245
anti-VPS53 antibody
anti-VPS53 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Polyclonal antibody recognizes VPS53 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VPS53 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human VPS53. |
| Conjugation | Un-conjugated |
| Alternate Names | VPS53; VPS53 Subunit Of GARP Complex; HCCS1; Vacuolar Protein Sorting-Associated Protein 53 Homolog; Hepatocellular Carcinoma Suppressor 1; VPS53, GARP Complex Subunit; FLJ10979; Vacuolar Protein Sorting 53 Homolog (S. Cerevisiae); Vacuolar Protein Sorting 53 (Yeast); HVps53L; Pp13624; PCH2E |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q5VIR6 Human Vacuolar protein sorting-associated protein 53 homolog Swiss-port # Q8CCB4 Mouse Vacuolar protein sorting-associated protein 53 homolog |
|---|---|
| Gene Symbol | VPS53 |
| Gene Full Name | VPS53 Subunit Of GARP Complex |
| Background | Involved in endocytic recycling and retrograde transport, endosome to Golgi. Acts upstream of or within lysosomal transport. Located in several cellular components, including Golgi apparatus; perinuclear region of cytoplasm; and recycling endosome. Part of EARP complex and GARP complex. Implicated in pontocerebellar hypoplasia type 2E. [provided by Alliance of Genome Resources, Nov 2024] |
| Function | Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. [UniProt] |
| Cellular Localization | Endosome ; Golgi apparatus ; Membrane. [UniProt] |
| Calculated MW | 94 kDa |
| PTM | Acetylation ; Phosphoprotein. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45245 anti-VPS53 antibody IHC-P image
Immunohistochemistry: Human lung adenocarcinoma stained with ARG45245 anti-VPS53 antibody at 2 μg/ml dilution.
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ARG45245 anti-VPS53 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45245 anti-VPS53 antibody at 5 ug/ml dilution.
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ARG45245 anti-VPS53 antibody WB image
Western blot: HepG2 and MCF-7 stained with ARG45245 anti-VPS53 antibody at 0.5 μg/ml dilution.
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ARG45245 anti-VPS53 antibody FACS image
Flow Cytometry: U87 stained with ARG45245 anti-VPS53 antibody at 1 µg/10^6 cells dilution.
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ARG45245 anti-VPS53 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45245 anti-VPS53 antibody at 2 μg/ml dilution.
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ARG45245 anti-VPS53 antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45245 anti-VPS53 antibody at 2 μg/ml dilution.
