ARG64017

anti-VPS45 antibody

anti-VPS45 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VPS45
Tested Reactivity Hu
Predict Reactivity Ms, Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VPS45
Antigen Species Human
Immunogen C-ESSQVTSRSASRR
Conjugation Un-conjugated
Alternate Names VSP45A; VPS45A; VSP45; VPS45B; VPS54A; H1; hlVps45; H1VPS45; SCN5; h-VPS45; Vacuolar protein sorting-associated protein 45

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11311 Human VPS45

Swiss-port # Q9NRW7 Human Vacuolar protein sorting-associated protein 45

Background Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 65 kDa

Images (4) Click the Picture to Zoom In

  • ARG64017 anti-VPS45 antibody WB image

    Western Blot: Human Peripheral Blood Mononucleocyte lysate (35 µg protein in RIPA buffer) stained with ARG64017 anti-VPS45 antibody at 0.3 µg/ml dilution.

  • ARG64017 anti-VPS45 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64017 anti-VPS45 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64017 anti-VPS45 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64017 anti-VPS45 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64017 anti-VPS45 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64017 anti-VPS45 antibody at 5 µg/ml dilution followed by AP-staining.