ARG63867

anti-VPS37C antibody

anti-VPS37C antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VPS37C
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VPS37C
Antigen Species Human
Immunogen C-ETLKDKTLQELEELQ
Conjugation Un-conjugated
Alternate Names hVps37C; Vacuolar protein sorting-associated protein 37C; ESCRT-I complex subunit VPS37C

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 6 µg/ml
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55048 Human VPS37C

Swiss-port # A5D8V6 Human Vacuolar protein sorting-associated protein 37C

Background VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]).[supplied by OMIM, Mar 2008]
Research Area Signaling Transduction antibody
Calculated MW 39 kDa
PTM Phosphorylated by TBK1.

Images (3) Click the Picture to Zoom In

  • ARG63867 anti-VPS37C antibody WB image

    Western Blot: human duodenum lysate (35 µg protein in RIPA buffer) stained with ARG63867 anti-VPS37C antibody at 0.03g/ml dilution.

  • ARG63867 anti-VPS37C antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63867 anti-VPS37C antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63867 anti-VPS37C antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Prostate. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63867 anti-VPS37C antibody at 3.8 µg/ml dilution followed by AP-staining.