ARG63418
anti-VPS35 / MEM3 antibody
anti-VPS35 / MEM3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes VPS35 / MEM3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VPS35 / MEM3 |
| Antigen Species | Human |
| Immunogen | C-SPESEGPIYEGLIL |
| Conjugation | Un-conjugated |
| Alternate Names | Maternal-embryonic 3; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35; PARK17; MEM3; hVPS35 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96QK1 Human Vacuolar protein sorting-associated protein 35 Swiss-port # Q9EQH3 Mouse Vacuolar protein sorting-associated protein 35 |
|---|---|
| Background | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 92 kDa |
Images (3) Click the Picture to Zoom In
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ARG63418 anti-VPS35 / MEM3 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63418 anti-VPS35 / MEM3 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63418 anti-VPS35 / MEM3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63418 anti-VPS35 / MEM3 antibody at 8 µg/ml dilution followed by HRP-staining.
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ARG63418 anti-VPS35 / MEM3 antibody WB image
Western blot: 35 µg of Human cerebellum (A) and Mouse brain (B) lysates (in RIPA buffer) stained with ARG63418 anti-VPS35 / MEM3 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
