ARG63990

anti-VPS29 antibody

anti-VPS29 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VPS29
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application IHC-P
Specificity This antibody is expected to recognise both reported isoforms (NP_057310.1 and NP_476528.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VPS29
Antigen Species Human
Immunogen CTGNLCTKESYDYLK
Conjugation Un-conjugated
Alternate Names PEP11; Vacuolar protein sorting-associated protein 29; DC15; Vesicle protein sorting 29; hVPS29; PEP11 homolog; DC7

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51699 Human VPS29

Swiss-port # Q9UBQ0 Human Vacuolar protein sorting-associated protein 29

Background This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 21 kDa

Images (2) Click the Picture to Zoom In

  • ARG63990 anti-VPS29 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63990 anti-VPS29 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63990 anti-VPS29 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Uterus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63990 anti-VPS29 antibody at 3.8 µg/ml dilution followed by AP-staining.