ARG63432

anti-VPS29 antibody

anti-VPS29 antibody for Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VPS29
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application WB
Specificity This antibody is expected to recognise both human isoforms (represented by NP_057310.1; NP_476528.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VPS29
Antigen Species Human
Immunogen C-DDVKVERIEYKKP
Conjugation Un-conjugated
Alternate Names PEP11; Vacuolar protein sorting-associated protein 29; DC15; Vesicle protein sorting 29; hVPS29; PEP11 homolog; DC7

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51699 Human VPS29

Swiss-port # Q9UBQ0 Human Vacuolar protein sorting-associated protein 29

Background This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 21 kDa

Images (1) Click the Picture to Zoom In

  • ARG63432 anti-VPS29 antibody WB image

    Western Blot: Human Spleen lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63432 anti-VPS29 antibody at 0.1 µg/ml dilution.