ARG63417
anti-VPS28 antibody
anti-VPS28 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes VPS28 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize isoform 1 (NP_057292.1) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VPS28 |
| Antigen Species | Human |
| Immunogen | C-ESAYNAFNRFLHA |
| Conjugation | Un-conjugated |
| Alternate Names | ESCRT-I complex subunit VPS28; H-Vps28; Vacuolar protein sorting-associated protein 28 homolog |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UK41 Human Vacuolar protein sorting-associated protein 28 homolog |
|---|---|
| Background | This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 25 kDa |
Images (2) Click the Picture to Zoom In
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ARG63417 anti-VPS28 antibody WB image
Western Blot: Human Testis lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63417 anti-VPS28 antibody at 1 µg/ml dilution.
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ARG63417 anti-VPS28 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63417 anti-VPS28 antibody at 10 µg/ml dilution followed by HRP-staining.
