ARG63885
anti-VPS25 antibody
anti-VPS25 antibody for Western blot and Human,Rat
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes VPS25 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VPS25 |
| Antigen Species | Human |
| Immunogen | C-QPNVDTRQKQ |
| Conjugation | Un-conjugated |
| Alternate Names | EAP20; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25; Dermal papilla-derived protein 9; FAP20; Vacuolar protein-sorting-associated protein 25; hVps25; DERP9 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P0C0A1 Rat Vacuolar protein-sorting-associated protein 25 Swiss-port # Q9BRG1 Human Vacuolar protein-sorting-associated protein 25 |
|---|---|
| Background | VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 21 kDa |
Images (2) Click the Picture to Zoom In
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ARG63885 anti-VPS25 antibody IHC-P image
Immunohistochemistry: Human kidney stained with ARG63885 anti-VPS25 antibody at 6 µg/ml dilution.
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ARG63885 anti-VPS25 antibody WB image
Western blot: 35 µg of Rat kidney lysate (in RIPA buffer) stained with ARG63885 anti-VPS25 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
