ARG45481
anti-VPRBP antibody
anti-VPRBP antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes VPRBP |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VPRBP |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human VPRBP. |
| Conjugation | Un-conjugated |
| Alternate Names | DCAF1; DDB1 And CUL4 Associated Factor 1; KIAA0800; VPRBP; Serine/Threonine-Protein Kinase VPRBP; DDB1- And CUL4-Associated Factor 1; Vpr (HIV-1) Binding Protein; HIV-1 Vpr-Binding Protein; Vpr-Interacting Protein; MGC102804; RIP; Vpr-Binding Protein; Protein VPRBP; EC 2.7.11.1; VprBP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 169 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DCAF1 |
| Gene Full Name | DDB1 And CUL4 Associated Factor 1 |
| Background | Enables histone H2AT120 kinase activity; nuclear estrogen receptor binding activity; and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including cell competition in a multicellular organism; proteasome-mediated ubiquitin-dependent protein catabolic process; and regulation of primary metabolic process. Located in COP9 signalosome; fibrillar center; and nucleoplasm. Part of Cul4-RING E3 ubiquitin ligase complex. Is active in centrosome. [provided by Alliance of Genome Resources, Feb 2025] |
| Function | Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. [UniProt] |
| Cellular Localization | Cytoplasm; Cytoskeleton; Nucleus. [UniProt] |
| Calculated MW | 169 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45481 anti-VPRBP antibody IHC-P image
Immunohistochemistry: Human chronic tonsillitis stained with ARG45481 anti-VPRBP antibody at 2 μg/ml dilution.
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ARG45481 anti-VPRBP antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45481 anti-VPRBP antibody at 5 μg/ml dilution.
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ARG45481 anti-VPRBP antibody WB image
Western blot: HEK293 stained with ARG45481 anti-VPRBP antibody at 0.5 μg/ml dilution.
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ARG45481 anti-VPRBP antibody FACS image
Flow Cytometry: 293T stained with ARG45481 anti-VPRBP antibody at 1 µg/10^6 cells dilution.
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ARG45481 anti-VPRBP antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45481 anti-VPRBP antibody at 2 μg/ml dilution.
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ARG45481 anti-VPRBP antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45481 anti-VPRBP antibody at 2 μg/ml dilution.
