ARG65165

anti-VLCAD antibody

anti-VLCAD antibody for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes VLCAD
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognize both reported isoforms (NP_000009.1; NP_001029031.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name VLCAD
Antigen Species Human
Immunogen DKSDSHPSDALTRK-C
Conjugation Un-conjugated
Alternate Names Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; EC 1.3.8.9; LCACD; ACAD6; VLCAD

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 37 Human ACADVL

Swiss-port # P49748 Human Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

Background The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 70 kDa
PTM S-nitrosylation at Cys-237 in liver improves catalytic efficiency.

Images (1) Click the Picture to Zoom In

  • ARG65165 anti-VLCAD antibody WB image

    Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65165 anti-VLCAD antibody at 2 µg/ml dilution.