ARG52467

anti-VGAT antibody

anti-VGAT antibody for Western blot and Rat

Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes VGAT
Tested Reactivity Rat
Predict Reactivity Bov, Dog, NHuPrm
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VGAT
Antigen Species Rat
Immunogen Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Conjugation Un-conjugated
Alternate Names Vesicular GABA transporter; GABA and glycine transporter; Vesicular inhibitory amino acid transporter; VIAAT; hVIAAT; VGAT; Solute carrier family 32 member 1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~53k VGAT protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 83612 Rat SLC32A1

Swiss-port # O35458 Rat Vesicular inhibitory amino acid transporter

Gene Symbol SLC32A1
Gene Full Name solute carrier family 32 (GABA vesicular transporter), member 1
Background The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
Highlight Related products:
Anti-Rabbit IgG secondary antibodies;
Related news:
Neuronal Development Marker
Research Area Neuroscience antibody
Calculated MW 57 kDa

Images (1) Click the Picture to Zoom In

  • ARG52467 anti-VGAT antibody WB image

    Western Blot: rat olfactory bulb lysate showing specific immunolabeling of the ~53k VGAT protein stained with VGAT antibody (ARG52467).