ARG22249
anti-VDAC1 / Porin antibody [S152B-23]
anti-VDAC1 / Porin antibody [S152B-23] for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [S152B-23] recognizes VDAC1 / Porin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, WB |
| Specificity | Detects ~30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S152B-23 |
| Isotype | IgG2a |
| Target Name | VDAC1 |
| Antigen Species | Human |
| Immunogen | Full-length (aa. 1-283) fusion protein of Human VDAC1. Mouse: 98% identity (279/283 around aa. identical). Rat: 98% identity (279/283 around aa. identical); >60% identity with VDAC2 and VDAC3. |
| Conjugation | Un-conjugated |
| Alternate Names | VDAC1; Voltage Dependent Anion Channel 1; PORIN; Voltage-Dependent Anion-Selective Channel Protein 1; Outer Mitochondrial Membrane Protein Porin 1; Plasmalemmal Porin; Porin 31HL; Porin 31HM; MGC111064; VDAC-1; Sperm Binding Protein 1a; HVDAC1; VDAC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol |
| Preservative | 0.1% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VDAC1 |
| Gene Full Name | voltage-dependent anion channel 1 |
| Background | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010] |
| Function | Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. [UniProt] |
| Cellular Localization | Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane. [UniProt] |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 31 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG22249 anti-VDAC1 / Porin antibody [S152B-23] WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG22249 anti-VDAC1 / Porin antibody [S152B-23] at 1:1000 dilution.
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ARG22249 anti-VDAC1 / Porin antibody [S152B-23] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22249 anti-VDAC1 / Porin antibody [S152B-23] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22249 anti-VDAC1 / Porin antibody [S152B-23] (D) Composite.
Customer's Feedback
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Review for anti-VDAC1 / Porin antibody [S152B-23]
Application：WB
Sample：Mouse brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
