ARG40751

anti-VARS / valyl tRNA synthetase antibody

anti-VARS / valyl tRNA synthetase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes VARS / valyl tRNA synthetase
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VARS / valyl tRNA synthetase
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-280 of Human VARS (NP_006286.1).
Conjugation Un-conjugated
Alternate Names Valyl-tRNA synthetase; G7A; EC 6.1.1.9; VARS2; VARS1; Valine--tRNA ligase; Protein G7a; ValRS

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver
Observed Size 140 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22321 Mouse VARS

GeneID: 25009 Rat VARS

GeneID: 7407 Human VARS

Gene Symbol VARS
Gene Full Name valyl-tRNA synthetase
Background Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]
Calculated MW 140 kDa

Images (2) Click the Picture to Zoom In

  • ARG40751 anti-VARS / valyl tRNA synthetase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG40751 anti-VARS / valyl tRNA synthetase antibody at 1:100 dilution.

  • ARG40751 anti-VARS / valyl tRNA synthetase antibody WB image

    Western blot: 25 µg of Mouse liver lysate stained with ARG40751 anti-VARS / valyl tRNA synthetase antibody at 1:1000 dilution.