ARG22239

anti-VAChT antibody [S6-38]

anti-VAChT antibody [S6-38] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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Overview

Product Description Mouse Monoclonal antibody [S6-38] recognizes VAChT
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Specificity Detects ~56kDa.
Host Mouse
Clonality Monoclonal
Clone S6-38
Isotype IgG1
Target Name VAChT
Antigen Species Human
Immunogen Synthetic peptide around aa. 521-532 of Human VAChT
Conjugation Un-conjugated
Alternate Names VAChT; VACHT; Vesicular acetylcholine transporter; Solute carrier family 18 member 3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC-P1:200
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 60422 Rat SLC18A3

GeneID: 6572 Human SLC18A3

Swiss-port # Q16572 Human Vesicular acetylcholine transporter

Swiss-port # Q62666 Rat Vesicular acetylcholine transporter

Gene Symbol SLC18A3
Gene Full Name solute carrier family 18 (vesicular acetylcholine transporter), member 3
Background This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008]
Function Involved in acetylcholine transport into synaptic vesicles. [UniProt]
Cellular Localization Membrane
Calculated MW 57 kDa

Images (2) Click the Picture to Zoom In

  • ARG22239 anti-VAChT antibody [S6-38] ICC/IF image

    Immunofluorescence: SH-SY5Y stained with ARG22239 anti-VAChT antibody [S6-38] at 1:200 dilution.

  • ARG22239 anti-VAChT antibody [S6-38] WB image

    Western blot: Rat brain membrane lysate stained with ARG22239 anti-VAChT antibody [S6-38] at 1:1000 dilution.

Specific References

Electroacupuncture improves cardiac function and reduces infarct size by modulating cardiac autonomic remodeling in a mouse model of myocardial ischemia

WB / Mouse

Sheng-Feng Lu et al.
Acupunct Med.,  (2021)

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