ARG62340
anti-V5 tag antibody [V5.E10]
anti-V5 tag antibody [V5.E10] for Dot blot,ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [V5.E10] recognizes V5 tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | Recognizes V5-tagged fusion proteins |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | V5.E10 |
| Isotype | IgG1 |
| Target Name | V5 tag |
| Antigen Species | Others |
| Immunogen | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A Purified |
| Purification Note | Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor. |
| Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). Both sequence is rarely be found in other proteins and can be used for protein analysis including binding assay, functional analysis and tracing the protein location in cells when the specific antibodies for the protein-of-interest are unavailable. |
|---|---|
| Highlight | Related products: V5 tag antibodies; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Gene Regulation antibody |
Images (2) Click the Picture to Zoom In
-
ARG62340 anti-V5 tag antibody [V5.E10] WB image
Western blot: 10 µg of 293T and 293T + myc-V5-mCherry cell lysates stained with ARG62340 anti-V5 tag antibody [V5.E10] at 1:1000 dilution.
-
ARG62340 anti-V5 tag antibody [V5.E10] IHC image
Immunohistochemistry: Zebrafish Embryos fixed with 0.4% Triton X-100 containing 4% paraformaldehyde and then stained with ARG62340 anti-V5 tag antibody [V5.E10] at 1:250 dilution.
Customer's Feedback
Excellent
Review for anti-V5 tag antibody [V5.E10]
Application：WB
Sample：293T and 293T + myc-V5-mCherry
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
