ARG40419
anti-Urokinase / uPA antibody
anti-Urokinase / uPA antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Urokinase / uPA |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Urokinase / uPA |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Urokinase / uPA. |
| Conjugation | Un-conjugated |
| Alternate Names | ATF; uPA; U-plasminogen activator; BDPLT5; EC 3.4.21.73; QPD; URK; Urokinase-type plasminogen activator; u-PA; UPA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P00749 Human Urokinase-type plasminogen activator Swiss-port # P06869 Mouse Urokinase-type plasminogen activator |
|---|---|
| Gene Symbol | PLAU |
| Gene Full Name | plasminogen activator, urokinase |
| Background | This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 49 kDa |
| PTM | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG40419 anti-Urokinase / uPA antibody WB image
Western blot: LNCaP cell lysate stained with ARG40419 anti-Urokinase / uPA antibody.
